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STEMCELL Technologies Inc easysep human cd8+ t cell enrichment kits
Easysep Human Cd8+ T Cell Enrichment Kits, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+enrichment+kit+human+cd8++t+cell/easysep+human+t+cell+isolation+kit/pm40300600-852-7-14
Average 90 stars, based on 1 article reviews
easysep human cd8+ t cell enrichment kits - by Bioz Stars, 2026-09
90/100 stars

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Cell Culture:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Selection:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Isolation:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Expressing:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Purification:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Flow Cytometry:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Knockdown:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Western Blot:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Negative Control:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Co-Culture Assay:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Cell Isolation:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.

Labeling:

Article Title: Therapeutic potential of T-cell receptor targeting the HLA-A*11:01-restricted KRASG12V neoantigen without cross-recognition of the self-antigen RAB7B in solid tumors
Article Snippet: Primary CD8 + T cells were enriched using the EasySep Human CD8 + T Cell Enrichment Kit (STEMCELL Technologies).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment.
Article Snippet: CD8+ T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8+ T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Autologous tumor organoid and immune cell co-cultures and methods of use as predictive models for pancreatic cancer treatment
Article Snippet: The resulting peripheral blood mononuclear cells (PBMCs) were cultured in human monocyte media, human dendritic cell media, or put through the negative selection EasySep Human CD8+ T cell Enrichment Kit (Stemcell).

Article Title: A Participant-Derived Xenograft Mouse Model to Decode Autologous Mechanisms of HIV Control and Evaluate Immunotherapies
Article Snippet: EasySep TM Human Memory CD8+ T-Cell Enrichment kit (STEMCELL Technologies, catalog number: 19159) 17.

Article Title: Leveraging Vγ9Vδ2 T cells against prostate cancer through a VHH-based PSMA-Vδ2 bispecific T cell engager
Article Snippet: M1-specific and pp65-specific CD8 + T cells were isolated from PBMC of CMV + and influenza + experienced donors (Sanquin) using an enrichment step (EasySep human CD8 + T cell isolation kit, 17953, STEMCELL Technologies) and subsequent sorting of CD8 + T cells using a BV510 labeled CD8α antibody ( ) and the A∗02:01-GILGFVFTL-dextramer (WB2161-APC, Immudex) for M1-specific CD8 + T cells or the A∗02:01-NLVPMVATV-dextramer (WB2132-APC, Immudex) for pp65-specific CD8 + T cells.

Article Title: Distinctive CD8 + T cell activation by antigen-presenting plasmacytoid dendritic cells compared to conventional dendritic cells.
Article Snippet: CD8+ T cells were enriched from CD34 or CD34 CD14-depleted PBMCs by negative magnetic bead selection according to the manufacturer’s instructions (EasySep Human CD8+ T cell Enrichment Kit, STEMCELL Technologies Cat#19053).

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment
Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Article Title: Loss of miR-200c-3p promotes resistance to radiation therapy via the DNA repair pathway in prostate cancer.
Article Snippet: Peripheral blood mononuclear cells were obtained from healthy donors (ethics agreement CPDL-PLER-2018 021), and CD8+ fraction was immediately isolated using EasySep Human CD8+ T cell Enrichment Kit (Stemcell, Cat# 19159) according to the manufacturer’s protocol.



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( a,b ) Cell clustering and identification by UMAP. ( c,d ) The heatmap and box plot of expression of SLC2A3 in identified subpopulations. ( e ) Purified CD8 + T cells from HNSC tissue were assayed by flow cytometric analysis. ( f ) Differential analysis of mRNA and protein expression between purified CD8 + T cells and other cell types in HNSC tissues.

Journal: Scientific Reports

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment

doi: 10.1038/s41598-024-79417-9

Figure Lengend Snippet: ( a,b ) Cell clustering and identification by UMAP. ( c,d ) The heatmap and box plot of expression of SLC2A3 in identified subpopulations. ( e ) Purified CD8 + T cells from HNSC tissue were assayed by flow cytometric analysis. ( f ) Differential analysis of mRNA and protein expression between purified CD8 + T cells and other cell types in HNSC tissues.

Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Techniques: Expressing, Purification

( a ) CD8 + T cells purified and activated from PBMCs were identified by flow cytometry(single/live/CD8 + /PD-1 - /Lag-3 - /Tim-3 − /Granzyme b + ). ( b ) Activated CD8 + T cells knockdown on SLC2A3 were assay by PCR and Western blot. ( c ) Knockdown of SLC2A3 and negative control of CD8 + T cell in CCK8 proliferation assessment. ( d ) The proliferation curve of CCK8 in a co-culture system. Two pairs were compared at the same time point. (TU686 vs TU686+ CD8 + T cell(si-NC), TU686+ CD8 + T cell(si-NC) vs TU686+ CD8 + T cell(si-SLC2A3)). ( e ) GSEA and Western blot were used to analyze ferroptosis in CD8 + T cells.

Journal: Scientific Reports

Article Title: SLC2A3 promotes head and neck squamous cancer developing through negatively regulating CD8 + T cell in tumor microenvironment

doi: 10.1038/s41598-024-79417-9

Figure Lengend Snippet: ( a ) CD8 + T cells purified and activated from PBMCs were identified by flow cytometry(single/live/CD8 + /PD-1 - /Lag-3 - /Tim-3 − /Granzyme b + ). ( b ) Activated CD8 + T cells knockdown on SLC2A3 were assay by PCR and Western blot. ( c ) Knockdown of SLC2A3 and negative control of CD8 + T cell in CCK8 proliferation assessment. ( d ) The proliferation curve of CCK8 in a co-culture system. Two pairs were compared at the same time point. (TU686 vs TU686+ CD8 + T cell(si-NC), TU686+ CD8 + T cell(si-NC) vs TU686+ CD8 + T cell(si-SLC2A3)). ( e ) GSEA and Western blot were used to analyze ferroptosis in CD8 + T cells.

Article Snippet: CD8 + T-cells were negatively isolated from PBMCs or clinical HNSC tumor tissues by magnetic separation using the EasySep Human CD8 + T-Cell Enrichment Kit (Stemcell Technologies, USA).

Techniques: Purification, Flow Cytometry, Knockdown, Western Blot, Negative Control, Co-Culture Assay